749 Transcriptome analysis of dorsal root ganglion in atopic dermatitis-induced mice reveals potential biomarker for atopic dermatitis

Itch is a typical symptom that exacerbates atopic dermatitis (AD) and associated with both the central peripheral nervous systems. In previous study, pruritogen mediators (histamine, serotonin, substance P, interleukin 31, thymic stromal lymphopoietin) secreted in skin bind to their receptors located on somatosensory neurons transmit itch signals brain. this we aimed elucidate profile of dorsal root ganglion (DRG), which contains cell bodies sensory neurons, from AD mice compared DRG control mice. We performed RNA seq analyses used DESeq2 Caret R packages for differentially expressed gene (DEG) random forest analyses, respectively. A total 142 DEGs between severe group were identified. These changed genes mainly enriched GO function related positive regulation inflammatory response, leukocyte migration involved adhesion. Also, 87 mild cellular oxidant detoxification. particular, brain-derived neurotrophic factor (Bdnf) stimulator interferon (Sting) most distinctly upregulated group. Moreover, expressions these gradually increased order control, mild, Collectively, found significant difference expression profiles comparisons mice, as well Furthermore, biomarkers such Bdnf Sting could be targetable suppress progression its symptoms.